Jong Bong Lee
Pohang University of Science and Technology
研究室紹介
Professor Jong Bong Lee's research lab specializes in single-molecule biophysics, focusing on developing advanced optical and mechanical techniques to visualize and quantify the dynamic behaviors of biomolecules, particularly DNA-processing enzymes. The lab pioneers innovative single-molecule assays, such as multiplexed force spectroscopy and flow-stretching methods, to study real-time enzymatic activities with high spatiotemporal resolution. Their work emphasizes understanding the mechanical and kinetic properties of protein-DNA complexes, revealing transient interactions and molecular heterogeneity in DNA metabolism. The lab integrates custom optical microscopy, nanomechanical measurements, and quantitative analysis to probe fundamental biological processes at the single-molecule level.
Research Overview
Research Output Trend
Figures are computed from collected data and may differ slightly.
Selected Papers
3Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high spatiotemporal resolutions of ∼nanometers and ∼milliseconds. However, DNA metabolism results from coordination of a number of components during th
Single-molecule force spectroscopy often refers to the measurement of the enzymatic activities coupled to the mechanical properties of biomolecules. We apply a simple dark-field microscope to a single-molecule flow-stretching method that quantitatively measures thermal fluctuations of the micron bead linked to DNA under a hydrodynamic force. Using various lengths of DNA attached to the bead, we measured the external force exerted on DNA immobilized on the surface and quantitatively showed the an