Jong-pyo Lim
Yonsei University · 生化学・遺伝学・分子生物学
研究室紹介
Professor Jong-pyo Lim's research lab specializes in clinical microbiology, infectious disease immunology, and biomarker discovery for cancer and renal dysfunction. The lab investigates antibiotic resistance mechanisms in pathogenic bacteria such as *Pseudomonas* species, explores host immune responses to viral infections like cytomegalovirus and human papillomavirus, and identifies novel diagnostic and therapeutic targets for gastric and cervical cancers using immunological and molecular techniques. The lab also focuses on improving renal function assessment through novel biomarkers such as cystatin C, aiming to enhance early detection and management of kidney disease.
Research Overview
Research Output Trend
Figures are computed from collected data and may differ slightly.
Selected Papers
11We investigated the phenotypic and genetic properties of metallo-beta-lactamase-producing Pseudomonas isolates collected at a tertiary-care hospital in Korea since 1995. The prevalence of imipenem resistance among Pseudomonas aeruginosa isolates reached 16% in 1997, when 9% of the resistant organisms were found to produce VIM-2 beta-lactamase, a class B enzyme previously found only in P. aeruginosa isolates from Europe. VIM-2-producing isolates of Pseudomonas putida were also detected. Resistanc
Purpose: We aimed to evaluate the clinical significance of a disintegrin and metalloproteinase 8 (ADAM 8) as a potential bloodbiomarker for gastric cancer (GC). Materials and Methods: Blood ADAM 8 was measured by ELISA. Cytokines/chemokines [interleukin-23 (IL-23), stromal cell-derivedfactor 1α/CXC chemokine ligand 12 (SDF-1α/CXCL12), interleukin-8 (IL-8), and soluble CD40 ligand (sCD40L)] were measuredby chemiluminescent immunoassay. They were compared among five groups; normal/gastritis, high-r
A human papilloma virus (HPV) test can be used to identify women that are at risk for cervical cancer. Hybrid Capture (HC2) and PCR are the current gold standard assays and are used globally to detect HPV infection. We present a combined assay system that uses PCR and fluorescence (FL) DNA chip for simple, rapid, and sensitive HPV DNA detection. In the developed assay system, PCR-amplified DNA produced by biotin-labeled consensus GP5+/GP6+ primers was denatured, neutralized, mixed with FL-strept
Cytomegalovirus (CMV) reactivation in immune compromised patients such as those undergoing hematopoietic progenitor cell transplantation (HPCT) and those with HIV infections can cause severe morbidity and mortality despite treatment with appropriate antiviral agents. The recovery of Cytomegalovirus (CMV) specific cytotoxic T lymphocytes (CTL) plays an important role in the reconstitution of CMV specific immunity in immunocompromised patients. Recent studies have reported that CMV reactivation ca
Purpose: To identify new immunogenic HLA-A*33;03-restricted epitopes from the human papillomavirus (HPV) 16 E7 proteinfor immunotherapy against cervical cancer. Materials and Methods: We synthesized fourteen overlapping 15-amino acid peptides and measured intracellular interferon-γ(IFN-γ) production in PBMC and CD8+ cytotoxic T lymphocytes (CTLs) after sensitization with these peptides using flow cytometryand ELISpot assay. The immunogenicity of epitopes was verified using a 51Cr release assay w
목 적 : 사구체 여과율은 신손상 정도와 진행을 예측하는데 중요한 척도이다. 현재까지는 혈청 creatinine을 광범위하게 사용하고 있으나 creatinine의 많은 단점으로 인해 새로운 내부표지자의 필요성이 대두되었고 cystatin C가 새로운 신기능 지표로 주목을 받고 있다. 이에 본 연구는 cystatin C의 유용성을 확인하고자 creatinine과 비교하여 연구를 진행하였다. 방 법 : 2004년 8월부터 2006년 12월까지 연세대학교 세브란스 병원 소아과를 내원한 환자들로서 다양한 신질환을 가지고 있는 43명(총 검사 횟수는 60회)을 대상으로 하였으며 creatinine, cystain C, creatinine 청소율을 측정하였고 creatinine 청소율이 감소된 환자를 그 정도에 따라 3군(Group 1 : CrCl < 40mL/min/1.73m2, Group 2 : CrCl 40-60 mL/min/1.73m2, Group 3 : CrCl > 60 mL/min/
배 경 : 혈관통로 (vascular access)의 기능부전은 투석환자에서 이환율의 가장 중요한 원인이다. 최근 MIA (Malnutrition, Inflammation, Atherosclerosis) syndrome이 알려지면서 투석환자에서 죽상경화증 (atherosclerosis)과 염증간의 관련성이 부각되었다. 혈액투석환자들의 혈관통로 협착과 죽상경화증의 조직학적 유사성을 생각할 때 염증매개성 물질에 의한 만성염증반응이 죽상경화증을 촉진시키는 것처럼 혈관통로의 협착도 촉진시킬 수 있다는 가설을 세워볼 수 있다. 따라서 혈관통로협착이 혈청의 염증매개성 싸이토카인 (cytokine)과 관련성이 있는지 살펴보고자 하였다. 방 법 : 혈액투석을 받는 환자 72명을 대상으로 하여 ELISA를 이용하여 interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ)의 혈청농도 및 염증표지자로써 C-반응단백, ferr
배경: 본 연구에서는 임상 화학 검사 현장 검사 기기인 Abbot사의 i-STAT CHEM8+를 이용하여 Na+, K+, Cl−, tCO2, BUN, Creatinine, Glucose, iCa를 포함하는 8가지 기본 화학 검사 항목에 대한 측정 능력을 평가하였다. 방법: 상기 8가지 항목에 대하여 CLSI의 기준을 이용하여 정밀도, 직선성을 평가하였고, 대형 검사 기기(Hitachi Clinical Analyzer 7600과 NOVA CCX)와 i-STAT CHEM8+ 간의 기기 간 상관성 평가를 실시하였다. 통계 분석은 Microsoft excel과 Analyse-it을 통하여 시행하였다. 결과: 검사차례내 정밀도와 총 정밀도의 표준편차는 Cl−를 제외한 7가지 항목에서 제조사에서 제시한 표준편차 또는 verification value 내로 우수한 결과를 보였다. 총 정밀도는 변이계수가 creatinine을 제외한 7가지 항목에서 2% 내로 우수한 결과를 보였다. 직선성 평가에서
Plasma hemoglobin (pHb) is measured when hemolysis is suspected as a result of biochemical, immunological, and mechanical conditions. In this study, we evaluated the clinical feasibility of the hemolysis index (H-index) for pHb measurement using automated chemistry analyzers. A total of 176 plasma samples were analyzed for pHb in the Severance Hospital using a Lambda 365 UV/Vis spectrophotometer (PerkinElmer, USA) and Fairbanks method 2. The remnant samples after pHb measurement were then analyz