Minee-Liane Choi

The dentate gyrus (DG) of the mammalian hippocampus is hypothesized to mediate pattern separation-the formation of distinct and orthogonal representations of mnemonic information-and also undergoes neurogenesis throughout life. How neurogenesis contributes to hippocampal function is largely unknown. Using adult mice in which hippocampal neurogenesis was ablated, we found specific impairments in spatial discrimination with two behavioral assays: (i) a spatial navigation radial arm maze task and (

RNA editing is a posttranscriptional mechanism that targets changes in RNA transcripts to modulate innate immune responses. We report the role of astrocyte-specific, ADAR1-mediated RNA editing in neuroinflammation in Parkinson's disease (PD). We generated human induced pluripotent stem cell-derived astrocytes, neurons and cocultures and exposed them to small soluble alpha-synuclein aggregates. Oligomeric alpha-synuclein triggered an inflammatory glial state associated with Toll-like receptor act

This protocol describes a method to perform calcium imaging with Fura-2 (live-cell imaging)

ABSTRACT Human induced pluripotent stem cells (hiPSCs) aim to replicate the developmental and functional capacity of human embryonic stem cells (hESCs). Here, we identify neuronal resilience under metabolic stress as a critical parameter for benchmarking equivalence. Without medium replenishment, hESC-derived cortical neurons underwent neural resurrection —a spontaneous recovery marked by increased cell density, preserved morphology, and sustained calcium signaling and mitochondrial function for

This protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.

This protocol describes a method of generating aggregation of human recombinant alpha-synuclein.

Abstract Mitochondrial dysfunction is a convergent hallmark of neurodegenerative diseases and represents a promising biomarker for early diagnosis and therapy. However, current in vitro assays rely on fluorescence or electron microscopy, which are invasive, low-throughput, and incompatible with longitudinal analysis. Here, we present a noninvasive framework by integrating label-free optical diffraction tomography (ODT) with organelle-aware representation learning to detect subtle mitochondrial d

This protocol contains instructions for examining cell death in live-cell imaging.

This is a modified protocol that describes how to generate cortical region-specific astrocytes based on Gupta et al., 2012, Serio et al., 2013 and Seto-Salvia et al., 2021

This protocol details the packaging of plasmids that are on a lentivirus backbone into a lentiviral construct, as well as cell transduction.

This protocol contains the instruction for measuring superoxide using dihydroethidium (HEt) which allows the rate of superoxide generation to be measured which is present as the ratio of the oxidized form of the dye over the reduced form. HEt allows the rate of cytosolic superoxide generation to be measured, by the slope of the ratio of oxidized dye to reduced dye.

This protocol details the packaging of plasmids that are on a lentivirus backbone into a lentiviral construct, as well as cell transduction.