엄창섭 교수

Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were ex

Helicobacter pylori specifically adheres to host cells, mainly based on carbohydrate-mediated cell-cell interactions. Previously, we investigated the anti-adhesive effect of polysaccharide fractions from Artemisia capillaris and Panax ginseng, using hemagglutination and enzyme-linked glycosorbent assays. In the present study, each active polysaccharide fraction was further purified, resulting in a single peak (fraction F2) using gel filtration FPLC, in which no protein content was detectable. Us

Null alpha1-antitrypsin (alpha1AT) alleles represent the end of a continuum of variants associated with profound alpha1AT deficiency and an increased risk of emphysema. This study characterizes the molecular basis of QOclayton, a new example of an alpha1AT null allele arising from a mutational hot spot in the alpha1AT gene. The QOclayton allele is identical to the normal M1(V213) alpha1AT allele except for an insertion of a cytosine. This insertion occurs in the alpha1AT sequence which normally

Proper sample preparation prior to microscopy is necessary for maintaining the components of tissues in a state as close to a living state as possible.For optimal preservation of biological samples, the sampling conditions are as important as the fixation itself.Various factors influence the selection and fixation efficiencies of a fixative, including sample size, osmolarity, pH, penetration rate and depth, fixative temperature, fixation time, fixative concentration, fixative amount, and retenti

OBJECTIVES/HYPOTHESIS: Laryngopharyngeal reflux (LPR) originates from regurgitation of gastric contents, a mechanism seemingly identical to gastroesophageal reflux disease (GERD). Some researchers postulate a connection between LPR and GERD, whereas some assert LPR is a disease apart from GERD. We examined symptoms of GERD from LPR patients, and performed gastrointestinal endoscopy and transmission electron microscopy (TEM) to evaluate GERD findings from these patients. STUDY DESIGN: Prospective

PURPOSE: To investigate the ultrastructures of tendon axonal profiles in infantile and intermittent exotropia. Tendon axonal profiles are composed of myotendinous nerve endings that are presumed to serve as sensorial receptors in ocular proprioception. METHODS: The study subjects included 10 patients with exotropia who had undergone surgery in one eye (recession and resection). They were divided into two equal groups. Five patients with infantile exotropia that had developed at under 12 months o

The therapeutic effect of safflower seed powder on experimental osteoporosis in the rat induced by ovariectomy was evaluated. Thirty Sprague-Dawley rats were ovariectomized at the age of 12 weeks. Seven weeks postovariectomy, rats were divided into two groups: control and safflower seed powder treated group. Five animals from each group were sacrificed at the following time points: 1, 3, and 5 weeks. Scanning electron microscopic observation and morphometric analysis of the tibiae epiphysis show

BACKGROUND AND AIMS: Bronchial asthma (BA) is considered an extra-esophageal syndrome of gastroesophageal reflux disease (GERD) with poor pathophysiological background. We analyzed the correlation between GERD and BA, examining esophageal epithelium with transmission electron microscopy (TEM), along with clinical findings. METHODS: BA patients of controlled and partly-controlled levels were enrolled in the study. A pulmonary and gastrointestinal (GI) questionnaire was given. Patients with no sym

Research ethics are mainly of two fields; research integrity and publication ethics. Research misconducts can occur at both areas. Examples of the research integrity violations are falsification, fabrication, and plagiarism; and those of the publication ethics violations are duplicate publication including self-plagiarism, and improper authorship. In this paper, concepts of research misconducts defined in Research Misconduct-related Rules of The Korean Society of Microscopy are explained and dis

The thickness of specimen is an important factor in microscopic researches.Thicker specimen contains more information, but it is diffi cult to obtain well focused image with precise details due to optical limit of conventional microscope.Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software.In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi staine

Peptide assimilation by Helicobacter pylori wasinvestigated using L-phenylalanyl-3-thia-phenylalanine (PSP)as a detector peptide; the release of thiophenol upon enzymatichydrolysis of PSP was spectrophotometrically detected withthe aid of 5,5'-dithiobis[2-nitrobenzoic acid] (DTNB). By addingPSP to whole-cell suspension, thiophenol was producedprogressively, resembling that found in Esherichia coli orStaphylococcus aureus. Interestingly, the rate of thiophenolproduction by H. pylori in particular

Safflower (Carthamus tinctorius L.) is a thistle-like annual plant mainly grown in dry hot climates as an oilseed or birdseed. Traditionally, the oil has mainly been sold in the health food market because it is unsaturated having high linoleic and oleic acid levels. With increased health consciousness in recent years, the oil quality has become a more general health issue. This study was designed to understand whether safflower seed powder has positive effects on the fracture healing in rats. Si

Bile canaliculi are the structure delivering bile secreted by hepatocytes into the bile passage. Bile secretion is mainly controlled by the cytoskeletal elements, mainly of actin in the microvilli, pericanalicular web. Most studies on the bile secretion have been done in viva situation, however, to control the various parameters in vitro culture system seem to be more useful. To set up an in vitro experimental system, the investigator isolated hepatocytes with an enzymatic method using a mixture