박계원 교수

The consumption of high-calorie foods combinedwith less physical exercise has increased the prevalenceof obesity. Obesity is also associated with high bloodpressure, atherosclerosis, insulin resistance, diabetes,impaired host defense, and the risk of some cancers. Because PPARc is a central player that participates invarious biological responses, including lipid metabolism,inflammation, and cell proliferation, further understandingof the lipid metabolic sensor PPARc is necessary to reducethe inc

Adipose tissue is a central metabolic organ that controls energy homeostasis of the whole body. White adipose tissue (WAT) stores excess energy in the form of triglycerides, whereas brown adipose tissue (BAT) dissipates energy in the form of heat through mitochondrial uncoupling protein 1 (Ucp1). A newly identified adipose tissue called ‘beige fat’ (BAT-like) is produced through a process called WAT browning. This tissue mainly resides in WAT depots and displays intermediate characteristics of b

Obesity is a leading risk factor for insulin resistance,hypertension, hyperlipidemia, and cardiovascular complications, collectively referred to as metabolic diseases. Given the prevalence of obesity and its associated medical problems, new strategies are required to prevent or treat obesity and obesity-related metabolic effects. Here we summarize contributors of obesity,and molecular mechanisms controlling adipogenesis from studies in mammalian systems. We also discuss the possibilities of usin

일반 느타리 13품종과 색상 느타리 5품종을 사용하여 아미노산 및 polyphenol, β-glucan 함량을 분석하고, 생리활성으로 항산화 및 항암, 항고혈압, 항혈전, 항당뇨, 항염활성을 측정하였다. 느타리버섯 18종의 아미노산 분석결과 전반적으로 감칠맛을 내는 glutamic acid 함량이 비교적 많이 함유되어 있었고, 필수아미노산 성분도 고르게 분포되어 있었다. Polyphenol 함량에서는 전품종에서 20 mg% 함량이상을 나타냈으며, 노랑느타리(R)가 39.13±0.82 mg%로 가장 높았다. β-glucan 함량은 노랑느타리(R)에서37.67±0.22%로 가장 높았으며, 그 외에 원형1(C), 장안PK(A)에서각각 28.75±0.61%, 27.95±0.33%의 순으로 나타났다. 전자공여능에서는 노랑느타리(R) 버섯의 DPPH IC50값이 2.93±0.44 mg/mL로가장 낮아 항산화 활성이 가장 우수한 것으로 나타났으며, 세포독성 실험에서는 노랑느타리(R) 에탄올 추출

We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcri

The bark of Prunus yedoensis is used in antitussive medicines and in oral herbal formulations for inflammatory skin disorders. In the present study, we explored whether P. yedoensis bark extract (PYE) and its solvent partitioned fractions could modulate lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-a and interleukin (IL)-6 in vivo and in vitro. In addition, we examined the effect of PYE extract and its fractions on LPS-induced NF-jB and mitogen-activated protein kinase (MAPK) sign

Previous studies showed that feeding diets containing the mature fruits of Sophora japonica L. prevented body weight gain and reduced fat mass in high-fat diet-induced obese mice. This observation has led to the hypothesis that extracts from S. japonica L. may inhibit adipocyte differentiation of preadipocytes. To elucidate the possible mechanisms for the anti-obesity action of S. japonica L., its effects on adipocyte differentiation were investigated in C3H10T1/2 mesenchymal stem cells and 3T3-

The antioxidant properties of roasted hulledbarley (Hordeum vulgare L.) were tested using in vitroassays, including the total phenolic content (TPC), totalflavonoid content (TFC), and DPPH, ABTS, and ferricreducing antioxidant power (FRAP) assays. Hulled barleywas roasted at 0, 170, 210, and 250oC for 20 min. Theeffects of the aqueous heating time at 100oC from 0 to 60min and the blending ratio of barley and water from 1:2 to1:5 in aqueous solutions were evaluated. p-Coumaric andferulic acid con

Chronic alcohol consumption contributes to numerous diseases,including cancers, cardiovascular diseases, and liver cirrhosis. Epidemiological studies have shown that excessive alcohol consumption is a risk factor for dementia. Along this line, Alzheimer’s disease (AD) is the most common form of dementia and is caused by the accumulation of amyloid-β (Aβplaques in neurons. In this study, we hypothesized that chronic ethanol consumption is associated with pathological processing of APP in AD. To i

Pathological increases in adipogenic potential with decreases in osteogenic differentiation occur in osteoporotic bone marrow cells. Previous studies have shown that bioactive materials isolated from natural products can reciprocally regulate adipogenic and osteogenic fates of bone marrow cells. In this study, we showed that Eupatorium japonicum stem extracts (EJE) suppressed lipid accumulation and inhibited the expression of adipocyte markers in multipotent C3H10T1/2 and primary bone marrow cel

결명자(Cassia obtusifolia) 및 포공영(Taraxacum platycarpum),유근피(Ulmus macrocarpa)로 제조된 생약재 추출혼합물이 in vitro 와 in vivo상에서 아토피 피부염에 미치는 영향을 실험하였다. HMC-1 cell을 이용하여 비만세포의 β-hexosaminidase 방출량을측정한 결과, 단일 추출물보다 추출복합물이 탈과립을 억제하는효과가 우수한 것으로 측정되었다. 그리고 HMC-1 cell에서 PMA 와 A23187로 자극하였을 때 생성되는 염증성 cytokine의 분비에서도 추출혼합물의 저해 효과가 가장 높게 나타났다. 아토피 질환모델인 NC/Nga 마우스를 사용하여 생약재 추출혼합물의 아토피 저감효과를 측정한 결과, 진드기 추출물에 의해 발진된 마우스의 귀두께 및 조직학적인 부종 관찰을 통해 생약재 추출혼합물 농도가 증가함에 따라 대조군에 비해 귀 두께 및 부종의 감소가 관찰되었다. 또한 마우스의 혈중 IgE 농도와 이와 관련된c

Petasites japonicus is a traditional medicine in Korea for treating carcinoma, bronchitis, and asthma. However, the molecular mechanisms of anti-cancer activity are not well understood. The purpose of this study was to confirm the anti-cancer activity of P. japonicus and its mechanism. Apoptosis was examined using morphological observation of nuclear changes and flow cytometry. Activation of apoptosis-related markers was detected. P. japonicus extract significantly inhibited HeLa cell growth in

During menopause, elderly woman experience physical and emotional symptoms, including hot flashes, night sweats, weight gain, vaginal dryness, and mood swings. In this study, we investigated the effects of mixed herbal extracts containing Angelica gigas Nakai and Cuscuta chinensis Lam. on menopause symptoms in ovariectomized (OVX) rats. Daily oral treatments of 500 mg/kg and 1,000 mg/kg in OVX rats for 8 weeks significantly reduced weight gain, inhibited hepatic lipid accumulation, and lowered s

ZAS3 is a large zinc finger transcription repressor that binds the κB-motif via two signature domains of ZASN and ZASC. A loss-of-function study showed that lack of ZAS3 protein induced accelerated cell proliferation and tumorigenesis. Conversely,gain-of-function studies showed that ZAS3 repressed NFκB-activated transcription by competing with NFκB for the κB-motif. Based on these observations, we hypothesize that ZAS3 promotes apoptosis by interrupting anti-apoptotic activity of NFκB. Here, we

Rice bran was heated at 120oC for 0 to 30 min to extend the oxidative stability. Also, effects of visible light irradiation on the crude rice bran oil (RBO) from rice bran heated at 120oC for 20 min were studied. As heat treatment time increased from 0 to 30 min, rice bran had significantly high oxidative stability at 40oC for 12 days (p<0.05). Headspace oxygen content in rice bran without heat treatment decreased significantly (p<0.05) whereas those in heat treated rice bran did not change sign