윤혜원 교수

Gliosarcoma (GS) is a rare variant (2%) of glioblastoma (GBM) that poses clinical genomic challenges because of its poor prognosis and limited genomic information. To gain a comprehensive view of the genomic alterations in GS and to understand the molecular etiology of GS, we applied whole-exome sequencing analyses for 28 GS cases (6 blood-matched fresh-frozen tissues for the discovery set, 22 formalin-fixed paraffin-embedded tissues for the validation set) and copy-number variation microarrays

Since 1990, many nucleic acid expression platforms consisting of DNA or RNA have been developed. However, although RNA expression platforms have been relatively neglected, several such platforms capped at the 5’ end of RNA by an anti-reverse cap analog have now been developed. At the same time, the capping reaction is a bottleneck in the production of such platforms, with high cost and low efficiency. Here, we investigated several viral and eukaryotic internal ribosome entry sites (IRESs) to dev

Abstract Since the specific accumulation of iodide in thyroid was found in 1915, radioiodine has been widely applied to diagnose and treat thyroid cancer. Iodide uptake occurs across the membrane of the thyroid follicular cells and cancer cells through an active transporter process mediated by the sodium iodide symporter (NIS). The NIS coding genes were cloned and identified from rat and human in 1996. Evaluation of the NIS gene and protein expression is critical in the management of thyroid can

Applications of nanotechnology in the medical field have provided the fundamentals of tremendous improvement in precise diagnosis and customized therapy. Recent advances in nanomedicine have led to establish a new concept of theragnosis, which utilizes nanomedicines as a therapeutic and diagnostic tool at the same time. The development of high affinity nanoparticles with large surface area and functional groups multiplies diagnostic and therapeutic capacities. Considering the specific conditions

이 연구는 충청남도 당진시의 탈석탄 에너지 전환 과정에서 나타난 정책적 배경과 행위자의 역할 및 이들 간의 상호작용 변화를 분석한다. 지역 에너지 전환에 대한 기존 연구들은 석탄화력과 원자력발전소, 송전선로 건설을 둘러싼 지역 갈등에 주목하며 갈등 전개와 관리방안에 관한 연구 위주로 진행되었다. 또한 중앙정부와 지자체를 포함하여 탈석탄 과정에 참여한 다양한 행위자의 역할과 이들 간의 상호작용에 대한 분석은 충분히 이루어지지 않았다. 따라서 이 연구에서는 정책네트워크 이론을 바탕으로 분석틀을 구성하여 문헌연구와 주요 행위자들에 대한 심층면접을 토대로 당진시의 탈석탄 에너지 전환 과정의 정책환경과 정책네트워크 변화를 분석한다. 아울러, 네트워크의 확장을 촉진하거나 저해하는 요인과 이를 주도하는 행위자를 분석 대상에 포함하여 관계 중심의 구조적인 분석을 진행함으로써, 탄소중립 달성을 위한 지역 에너지 전환에 있어서의 정책적 시사점을 도출한다. 연구 결과, 지역 에너지 전환을 위한 정책네트

Background: Graft-versus-host disease (GVHD) is a huddle for success of hematopoietic stem cell transplantation. In this study, effects of irradiation dose on immune kinetics of GVHD were investigated using B6 → BALB.B system, a mouse model for GVHD after MHC-matched allogeneic transplantation. Methods: BALB.B mice were transplanted with bone marrow and spleen cells from C57BL/6 mice after irradiation with different doses. Leukocytes residing in the peripheral blood and target organs were collec

In allogeneic transplantation, including the B6 anti-BALB.B settings, H60 and H4 are two representative dominant minor histocompatibility antigens that induce strong CD8 T-cell responses. With different distribution patterns, H60 expression isrestricted to hematopoietic cells, whereas H4 is ubiquitously expressed. H60-specific CD8 T-cell response has been known to be dominant in most cases of B6 anti-BALB.B allo-responses, except in the case of skin transplantation. To understand themechanism un

In order to develop a successful vaccine against deadly diseases with a wide range of antigenic diversity, an in-depth knowledge of the molecules and signaling mechanisms between the vaccine candidates and immune cells is required. Therefore, monitoring vaccine components, such as antigen or adjuvants, and immune cell dynamics at the vaccination site or draining lymph nodes can provide important information to understand more about the vaccine response. This review briefly introduces and describ

Purpose The heat shock protein 90 inhibitor, tanespimycin, is an anticancer agent known to increase iodine accumulation in normal and cancerous thyroid cells. Iodine accumulation is regulated by membrane proteins such as sodium iodide symporter (NIS) and pendrin (PDS), and thus we attempted to characterize the effects of tanespimycin on those genes. Methods Cells were incubated with tanespimycin in order to evaluate 125I accumulation and efflux ability. Radioiodine uptake and efflux were measure

Graft-versus-host disease (GVHD) results from immune-mediated attacks on recipient tissues by donor-originated cells through the recognition of incompatible antigens expressed on host cells. The pre-conditioning irradiation dose is a risk factor influencing GVHD severity. In this study, using newly generated luciferase transgenic mice on a B6 background (B6.LucTg) as bone marrow and splenocyte donors, we explored the effects of irradiation doses on donor cell dynamics in major histocompatibility

육상풍력발전 사업은 기후위기 대응을 위한 재생에너지 이용 확대와 산림 보호라는 녹색과 녹색 가치가 충돌하면서 입지 애로와 주민 수용성 문제를 겪고 있다. 이 연구에서는 주민 수용성이 확보되었음에도 입지 애로 문제로 인해 사업 추진이 지연되고 있는 육백산 풍력발전 사업을 중심으로 문헌연구와 심층면접을 통해 육상풍력발전 사업의 입지 관련 제도적 한계를 분석하고, 개선방안을 제안하는 것을 목적으로 한다. 연구 결과, 사업 추진 과정에서 다음의 세 가지 쟁점을 확인하였다. 우선, 생태자연도 등급 적용 시점이 명시되어 있지 않아 개발행위허가 과정에서 생태자연도 등급이 상향되는 경우 소급 적용되는 문제가 발생하고 있다. 다음으로 「육상풍력 개발사업 환경성평가 지침」의 모호성에 기인한 해석 차이로 인해 혼란이 야기되고 있으며, 마지막으로 개발행위허가 평가 가이드라인의 부재로 부처 간 행정절차 혼선이 빚어지고 있다. 이 연구에서는 세 가지 쟁점들을 토대로 다음과 같은 개선방안을 제시한다. 첫째,

My dissertation examines the emergence of photographic portraiture as a vehicle for illuminating the experience of European exiles and their cultural migrations under the threat of fascism. I anchor my study in the works of three women European émigrés, each of whom produced a series of portraits while in exile: the German-born French Gisèle Freund (1908-2000), the Austrian-born American Lisette Model (1910-1983), and the German-born American Lotte Jacobi (1896-1980). Despite different working t

Aleksandr Rodchenko (1891-1956), a leading revolutionary artist of the Soviet avant-garde, produced a series of three portrait photographs of his wife and fellow artist, Varvara Stepanova (1894-1958), during a family vacation in 1927. Although these portraits have received relatively little attention from both the public and critics, they employ the oblique angle to transform Stepanova’s face and body into strange, exaggerated, and childlike forms. This formal experiment can be understood as a v