황인아 교수

The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased with the incidence of obesity; however, the underlying mechanisms are unknown. In this study, high-resolution metabolomics (HRM) along with transcriptomics were applied on animal models to draw a mechanistic insight of NAFLD. Wild type (WT) and catalase knockout (CKO) mice were fed with normal fat diet (NFD) or high fat diet (HFD) to identify the changes in metabolic and transcriptomic profiles caused by catalase gene delet

Osteopontin (OPN)은 정상적으로 콩팥세관에서 분비되는 인단백질로, 그 역할 및 조절기전이 아직 명 확하게 알려져 있지 않다. 본 연구의 목적은 콩팥에서 탈수와 관련된 OPN의 발현변화를 관찰하는 것이다. Sprague-Dawley계의 흰쥐를 대상으로 3일간 물을 제한하고 대조군과 비교하였다. OPN에 대한 항체와 riboprobe를 이용하여 광학 및 전자현미경적 면역세포화학법과 in situ hybridization을 시행하였다. 탈수군에서는 대조군에 비해 혈중 Na+ 농도와 소변의 오스몰농도가 증가하였다. 대조군에서 OPN 단백질과 mRNA의 발현은 헨레고리의 내림가는부분 (descending thin limb)과 콩팥유두상피 (renal papillary surface epithelium)에만 국한되어 발현되었으나, 3일간의 탈수군에서는 내림가는부분과 유두상피 외에 헨레고리의 굵은 오름부분 (thick ascending limb)에서도 강하게 발현되었다. 전자현미경으

E-cadherin is a cell adhesion molecule that is expressed abundantly in the kidney. However, the expression pattern in various renal epithelial cells is not well established. The purpose of this study was to determine the cellular localization along the nephron segments in the rat kidney. Kidneys from adult Sprague Dawley rats were fixed in 4% paraformaldehyde and processed for immunohistochemistry. Bumetanide-sensitive Na+/K+/2Cl- cotransporter (BSC1), thiazide-sensitive Na+/Cl- cotransporter (T

Abstract YAP/TAZ, cofactors of TEA domain-containing transcription factor TEADs (TEAD1-4), play a key role in the HIPPO pathway, which is involved in various function including embryonic development, organ size, cell proliferation, tissue regeneration, and the development and progression of several cancers. YAP/TAZ is therefore considered as master regulators of various cancers. However, we are interested in VGLL1, a cofactor of TEAD4, because it is mainly involved in the proliferation and metas