윤지훈 교수

Purpose: The genus Aeromonas is a pathogen that is well known to cause severe clinical illnesses, ranging from gastroenteritis to sepsis. Accurate identification of A. hydrophila, A. caviae, and A. veronii is important for the care of patients. However, species identification remains difficult using conventional methods. The aim of this study was to compare the accuracy of different methods of identifying Aeromonas at the species level: a biochemical method, matrix-assisted laser desorption ioni

Hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, thus melanin synthesis inhibitors have been of great interest as target molecules for cosmetic and medicinal purposes. The aim of this study was to investigate the in vitro inhibitory effect of panduratin A, isolated from Kaempferia pandurata Roxb., on melanogenesis and its related enzymes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2) in melan-a murine me

Human cytochrome P450 2C9 is a highly polymorphic enzyme that is required for drug and xenobiotic metabolism. Here, we studied eleven P450 2C9 genetic variants—including three novel variants F69S, L310V, and Q324X—that were clinically identified in Korean patients. P450 2C9 variant enzymes were expressed in Escherichia coli and their bicistronic membrane fractions were prepared The CO-binding spectra were obtained for nine enzyme variants, indicating P450 holoenzymes, but not for the M02 (L90P)

BACKGROUND: While experimental models show that leptin and adiponectin have inverse effects on the cardiovascular system, it has been suggested that the leptin-to-adiponectin (L/A) ratio may be an important predictor of cardiovascular disease and death. Higher circulating leptin and adiponectin levels are observed in uremia due to decreased renal degradation and/or clearance and increased production. We sought to examine the association between the L/A ratio and mortality in a prospective hemodi

Abstract BACKGROUND: Coagulation analyzers based on turbidimetry provide clot waveform data. Clotting times are defined only a point on this curve and have limitations to represent the whole characteristics of curve. We investigated to represent the whole curve shape by additional parameters and explored its clinical utility. METHODS: We applied the Gompertz growth model to the waveform and the two-dimensional curve shape can be expressed with novel parameters. The model was evaluated in terms o

Background: Protein-energy wasting (PEW) is highly prevalent in end-stage kidney disease (ESKD) patients receiving hemodialysis (HD), and is a potent predictor of survival in this population. We sought to conduct Subjective Global Assessment (SGA) surveys, a simple yet validated nutritional assessment instrument, in a prospective cohort of HD patients in order to assess the relationship between PEW and survival in this population. Methods: In a multi-center prospective cohort of 1018 HD patients

Background: Advanced CKD patients transitioning to ESKD have high morbidity and mortality in the first few months of dialysis initiation. We sought to determine whether transitioning to dialysis in the inpatient vs. outpatient setting is associated with differential hospitalization outcomes among incident ESKD patients. Methods: We examined advanced CKD patients (≥2 eGFRs <25 separated by ≥90 days) who transitioned to dialysis within 2-yrs of their 1st (index) eGFR <25 over 1/1/07-6/30/20 from t

Background: Dialysis is linked with high early mortality, frequent hospitalizations, and high withdrawal in certain groups, catalyzing interest in conservative management (CM) as an alternative treatment option for advanced CKD. We conducted an interim analysis of the prospective NIH OPTIMAL cohort examining patient-centered outcomes in advanced CKD patients treated with CM vs. dialysis. Methods: We conducted an interim analysis of the prospective NIH OPTIMAL cohort comparing CM vs. dialysis pre

Background: Large population-based studies show advanced CKD patients transitioning to ESKD have high mortality in the first few months of dialysis initiation. It is unclear whether transitioning to dialysis in the inpatient vs. outpatient setting is associated with better survival in incident ESKD patients. Methods: We examined advanced CKD patients (≥2 eGFRs <25 separated by ≥90 days) who transitioned to within 2-years of their 1st (index) eGFR <25 over 1/1/07-6/30/20 from the Optum Labs Data

Background: Given that dialysis patients may experience high early mortality rates, healthcare utilization, and withdrawal, particularly in those of older age and multi-morbidity, there has been interest in conservative management (CM) as an alternative treatment strategy for advanced CKD patients. Among a national cohort of US Veterans, we compared the impact of CM vs. dialysis on survival. Methods: Using linked national VA, USRDS, and Medicare data, we examined Veterans with advanced CKD (≥2 e

Background: Leptin and adiponectin are two major adipocytokines believed to play key roles in the regulation of CV and metabolic status. While animal studies show leptin and adiponectin have inverse effects on the CV system (leptin promotes atherosclerosis, while adiponectin reduces vascular injury), it is suggested that the leptin-to-adiponectin (L/A) ratio may be an important predictor of CV disease and death. Despite their exceedingly high CV risk, no studies have examined the association bet

저자들은 수술 후 발열과 함께 복막염이 의심되는 환자에서 시행한 복막액 배양에서 세 건의 드문 Bacteroides 균종을 분리하였다. 세 균주들은 Rapid ID 32A (bioMérieux, France) 검사로 각각 B. fragilis/B. stercoris와 B. ovatus로 동정되었으나, MALDI-TOF MS와 16S rRNA 염기서열 분석을 통해 B. nordii 1예와 B. salyersiae 2예로 확인되었다. 세 균주는 항혐기성 약제인 piperacillin/tazobactam, imipenem, meropenem, chloramphenicol 및 metronidazole에 대해서 모두 감수성이었으며, 환자들은 piperacillin/tazobactam 치료 후 호전되었다. 본 증례를 통해 드문 B. fragilis군균종 동정시에 MALDI-TOF MS가 16S rRNA 염기서열 분석 결과와 일치도가 높고, 간편하고 신속하게 결과를 확인할 수 있다는 점에서 유용

Background: While dialysis has been the dominant treatment paradigm for ESKD, this approach may not offer survival benefit nor improved quality of life in certain subgroups (comorbid, elderly). We examined comorbidity trends in a contemporary cohort of US Veterans with advanced CKD treated with dialysis vs. conservative management (CM). Methods: In a national cohort of US Veterans, we examined patients with advanced CKD (≥2 eGFRs <25ml/min/1.73m2 separated by ≥90 days from 10/2010-9/2019). Using