박진하 교수

PURPOSE: To compare and evaluate HER-2/neu clinical assay methods. MATERIALS AND METHODS: One hundred seventeen breast cancer specimens with known HER-2/neu amplification and overexpression status were assayed with four different immunohistochemical assays and two different fluorescence in situ hybridization (FISH) assays. RESULTS: The accuracy of the FISH assays for HER-2/neu gene amplification was high, 97.4% for the Vysis PathVision assay (Vysis, Inc, Downers Grove, IL) and 95.7% for the the

PURPOSE: To critically assess the accuracy and reproducibility of human epidermal growth factor receptor type 2 (HER-2) testing in outside/local community-based hospitals versus two centralized reference laboratories and its effect on selection of women for trastuzumab (Herceptin)-based clinical trials. EXPERIMENTAL DESIGN: Breast cancer specimens from 2,600 women were prospectively evaluated by fluorescence in situ hybridization (FISH) for entry into Breast Cancer International Research Group (

Tracking stem cell localization, survival, differentiation, and proliferation after transplantation in living subjects is essential for understanding stem cell biology and physiology. In this study, we investigated the long-term stability of reporter gene expression in an embryonic rat cardiomyoblast cell line and the role of epigenetic modulation on reversing reporter gene silencing. Cells were stably transfected with plasmids carrying cytomegalovirus promoter driving firefly luciferase reporte

The goal of this study was to characterize the relationship between tumor uptake of ⁶⁴Cu-DOTA-trastuzumab as measured by PET/CT and standard, immunohistochemistry (IHC)-based, histopathologic classification of human epidermal growth factor receptor 2 (HER2) status in women with metastatic breast cancer (MBC). <b>Methods:</b> Women with biopsy-confirmed MBC and not given trastuzumab for 2 mo or more underwent complete staging, including ¹⁸F-FDG PET/CT. Patients were classifi

An engineered antibody fragment (minibody; scFv-C(H)3gamma(1) dimer, M(r) 80 000) specific for carcinoembryonic antigen (CEA) has previously demonstrated excellent tumor targeting coupled with rapid clearance in vivo. In this study, variable (V) genes from the anti- p185(HER-2) 10H8 antibody were similarly assembled and expressed. Four constructs were made: first, the V genes were assembled in both orientations (V(L)-linker-V(H) and V(H)-linker-V(L)) as single chain Fvs (scFvs). Then each scFv w

Microstructural investigations on a series of (Ti70.5Fe29.5)100−xSnx alloys with x=5, 7, and 9 reveal that Sn addition is effective in introducing both structural and spatial heterogeneities in ultrafine eutectic composites stemming from a large temperature difference between two eutectic temperatures upon solidification. The microstructural heterogeneities in these ultrafine eutectic composites strongly enhance the room temperature compressive plasticity up to ∼15.7%.

The influence of partial replacement of Fe by Ni in (Fe1−xNix)71Nb6B23 (0≤x≤0.5) bulk glassy alloys on the enhancement of the plasticity has been investigated. The increase of the Ni-to-Fe concentration ratio effectively improves the compressive plasticity. The (Fe0.5Ni0.5)71Nb6B23 alloy exhibits an enhanced plastic strain of ∼4.2%, together with high strength and distinct strain hardeninglike characteristics, when compared with the Ni-free Fe71Nb6B23 glass. The improved mechanical properties ar

BACKGROUND: Pancreatic cancer (PC) hijacks innate cellular processes to promote cancer growth. We hypothesized that PC exploits PD-1/PD-L1 not only to avoid immune responses, but to directly enhance growth. We also hypothesized that immune checkpoint inhibitors (ICIs) have direct cytotoxicity in PC. We sought to elucidate therapeutic targeting of PD-1/PD-L1. METHODS: PD-1 was assessed in PC cells, patient-derived organoids (PDOs), and clinical tissues. Then, PC cells were exposed to PD-L1 to eva

The imaging of molecular events in the complex physiological interplay between organelles, cells, tissues, and organs in the whole organism is now more practical through the use of small-animal imaging technologies. Radionuclide based molecular imaging using single photon emission computed tomography (SPECT) and positron emission tomography (PET) scanners have been used for imaging intracellular enzymes, receptors, transporters, reporter gene expression, and cell trafficking in small animals suc

A heterostructured Fe71Si15Ti10Cu4 ultrafine composite has been developed. The addition of Cu with large positive enthalpy of mixing with the main constituent element (Fe) leads to evolution of a unique microstructure with different length-scale heterogeneities, i.e., formation of spherical colonies containing length-scale heterogeneity of lamellar spacing and precipitation of nanoscale multitwinned Cu within α-Fe layers. These macroscopic and microscopic structural heterogeneities are able to s

Novel therapies that require internalization of effector domains may be improved by assessing the efficacy of postbinding receptor-mediated endocytosis. To achieve targeted gene therapy of immunotoxin therapy, natural vector-host tropisms must be altered. Recent improvements in monoclonal antibody (MAb) engineering have expanded the potential range of host cells that can be targeted for therapeutic intervention. However, relatively little is known about cellular responses after binding of a vect