김종원 교수

The relationship between NexLN and survival is probably affected by stage migration in a high-volume gastric cancer center. The LN ratio system could be a better option to compensate for this effect, and the value of the prognosis prediction in this system increases with a higher NexLN.

CONTEXT: Detection of the BRAF V600E mutation in fine-needle aspiration cytology (FNAC) specimens may increase the value of FNAC. OBJECTIVE: The objectives of the study was to compare the diagnostic performance of BRAF assays that differ in sensitivity and to examine the associations between the BRAF V600E mutation status and the clinicopathological features in papillary thyroid carcinoma (PTC). DESIGN AND SETTING: Three molecular assays were performed in all subjects and compared with regard to

Chinese hamster ovary (CHO) cells are the most common cell line used for the production of therapeutic proteins including monoclonal antibodies (mAbs). Host cell proteins (HCPs), secreted and released from lysed cells, accumulate extracellularly during the cultures of recombinant CHO (rCHO) cells, potentially impairing product quality. In an effort to maintain good mAb quality during the cultures, HCPs accumulated extracellularly in batch and fed-batch cultures of a mAb-producing rCHO cell line

Saponins are natural compounds found in plants and have a diverse range of applications. However, the therapeutic potential of saponins in regulating cytotoxicity, angiogenesis, and inflammation in mammalian cells is yet to be explored. Here, we investigated the therapeutic effects of saponins from green tea by exploring the cytotoxic effects of saponins by inducing apoptosis in the human cancer cell lines hepatocellular carcinoma (HEPG2) and colorectal adenocarcinoma (HT29). The anti-angiogenes

We could not convincingly replicate most of the previous studies, a result that is possibly due to modest association between the suggested genes. Rather, we found a new candidate gene, ABCB1, for treatment response, which may provide a hypothesis on the relationship between the blood-brain distribution of CLZ and its clinical efficacy.

Coalescence frequency and lifetimes of thin aqueous films formed between nitrogen bubbles were measured in dilute solutions of sodium lauryl sulfate, n-amyl alcohol, and 1-butanol. The bubble pairs were formed on two adjacent capillary tubes by use of a microprocessor-controlled stepping motor, and the coalescence time was determined by an optical sensing method. For low-molecular weight alcohols such as 1-butanol and n-amyl alcohol, the transition concentration decreased with increase of bubbli

The UGT1A7 gene encodes UDP-glucuronosyltransferase, a key enzyme catalyzing the glucuronidation of various carcinogens. In this study, we investigated the association between haplotypes of the whole UGT1A7 gene and the risk of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B. Sequence analysis of exon1 and the promoter region of the UGT1A7 gene was carried out to determine haplotype profiles for 244 patients with hepatocellular carcinoma, 223 hepatitis B carriers, and 314 hea

UDP-glucuronosyltransferase 1A1 (UGT1A1) is the key enzyme for bilirubin conjugation. Defects in this enzyme can cause a nonhemolytic unconjugated hyperbilirubinemia, such as Crigler–Najjar syndrome type 1 (CN1) and 2 (CN2) and Gilbert syndrome (GS). In 1991, the cDNA of the human UGT1A1 gene was cloned, and this led to the identification of genetic defects in patients with CN1, CN2, and GS (1)(2)(3). It was shown that homozygous or compound heterozygous mutations of the UGT1A1 gene can lead to

Gaucher Disease type 1, the most prevalent lysosomal disease among Caucasians, is due to defects in the activity of acid beta-glucosidase. Over 40 missense, nonsense, and more complex alleles have been described, primarily in Western populations. From these results, predictive genotype/phenotype correlations have been developed and used to guide genetic counseling and therapy. Only a few mutations have been described in Japanese patients with Gaucher disease and many of these have resulted in se

The important cellular proteins for 5-fluorouracil (5-FU) metabolism are the major target enzymes, thymidylate synthase, and the rate-limiting enzyme in the degradation pathway, dihydropyrimidine dehydrogenase. Adverse drug reactions to 5-FU-based chemotherapy have been reported to be in part the result of polymorphisms in the thymidylate synthase (TYMS) and dihydropyrimidine dehydrogenase (DPYD) genes. Therefore, we examined the type and frequency of polymorphisms in the TYMS and DPYD genes in

The plant alkaloid berberine has many biological activities including the ability to induce cell cycle arrest and apoptosis, making it a potentially useful agent for targeting cancer cells. We have analyzed the effects of berberine on MCF-7 breast cancer cells. Berberine was added to MCF-7 cells in culture, and proliferation, side population (SP) cells and expression of ABCG2 were examined. Berberine caused a dose-dependent reduction in proliferation. Hoechst 33,342 dye staining and FACS analysi