공경혜 교수

Although it is known that the expression and activity of sirtuin 1 (Sirt1) decrease in the aged kidney, the role of interaction between Sirt1 and hypoxia-inducible factor (HIF)-1α is largely unknown. In this study, we investigated whether HIF-1α could be a deacetylation target of Sirt1 and the effect of their interaction on age-associated renal injury. Five-week-old (young) and 24-month-old (old) C57Bl/6J mice were assessed for their age-associated changes. Kidneys from aged mice showed increase

Resveratrol is a phytoalexin and natural phenol that is present at relatively high concentrations in peanuts and red grapes and wine. Based upon studies of yeast and invertebrate models, it has been proposed that ingestion of resveratrol may also have anti-aging actions in mammals including humans. It has been suggested that resveratrol exerts its beneficial effects on health by activating the same cellular signaling pathways that are activated by dietary energy restriction (DR). Some studies ha

Hypoxia-inducible factor (HIF) is a key transcriptional factor in the response to hypoxia. Although the effect of HIF activation in chronic kidney disease (CKD) has been widely evaluated, the results have been inconsistent until now. This study aimed to investigate the effects of HIF-2α activation on renal fibrosis according to the activation timing in inducible tubule-specific transgenic mice with non-diabetic CKD. HIF-2α activation in renal tubular cells upregulated mRNA and protein expression

BACKGROUND: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. METHODS: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was a

In 2006, the Korea Food and Drug Administration reported that combinations of dietary colors such as allura red AC (R40), tartrazine (Y4), sunset yellow FCF (Y5), amaranth (R2), and brilliant blue FCF (B1) are widely used in food manufacturing. Although individual tar food colors are controlled based on acceptable daily intake (ADI), there is no apparent information available for how combinations of these additives affect food safety. In the current study, the potencies of single and combination

Capsaicin (N-vanillyl-8-methyl-1-nonenamide) is a major pungent ingredient in hot peppers and induces apoptosis in malignant carcinoma cell lines. However, the adverse effects of capsaicin on neuronal development have not been fully explored. The aim of this study was to determine whether capsaicin affected murine-derived cerebellar multi-potent neural progenitor cells (NPC) or adult hippocampal neurogenesis in vivo. Capsaicin dose-dependently suppressed NPC proliferation, and higher concentrati

Renal fibrosis is the final manifestation of chronic kidney disease (CKD) regardless of etiology. Hypoxia-inducible factor-2 alpha (HIF-2α) is an important regulator of chronic hypoxia, and the late-stage renal tubular HIF-2α activation exerts protective effects against renal fibrosis. However, its specific role in progressive renal fibrosis remains unclear. Here, we investigated the effects of the long-term tubular activation of HIF-2α on renal function and fibrosis, using in vivo and in vitro

<b>Purpose:</b> To compare the therapeutic effects of human derivatives in a mouse alkali burn model. <b>Methods:</b> The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 d: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3 degrees of opacity on days 2, 3, and 7, and corneal neovascularization (

In this study, we investigated whether p-anisaldehyde (PAA), the main component of essential oils derived from anise seeds, influences the differentiation of mouse C2C12 myoblasts.Cells were induced to differentiate over 5 days using a differentiation medium with or without PAA (50 or 200 mg/mL).Myotube length and diameter were measured, and the expressions of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain)

INTRODUCTION AND AIMS: Although hypoxia-inducible factor (HIF) is a key transcriptional factor in the response to hypoxia, and the effect of selective tubular activation of HIF-2α on renal fibrosis was demonstrated recently, there is no study for the effect of long-term tubular activation of HIF-2α on the progressive renal fibrosis. METHODS:: Using PAX8-rtTA/tetO-Cre/HIF2dPA-HA transgenic mice (Tg), we have already bred tubular HIF-2α overexpressive Tg mice which is induced by doxycycline (2mg/m

INTRODUCTION AND AIMS: Muscledamage (rhabdomyolysis)releases myoglobinintothebloodstream and may induce acute kidney injury (AKI). Once filtered by the kidney, myoglobin causes oxidative stress, tubular death and endothelial damage. There is no specific treatment for rhabdomyolysis, only palliative care. Curcumin, a compound from the Curcuma longa, is a powerful antioxidant because of its inducer effect on Nrf2 (Nuclear factor (erythroid-derived 2) -like 2). The purpose of this study is to analy

Renal fibrosis is the final manifestation of chronic kidney disease(CKD) regardless of etiology. Hypoxia-inducible factor-2 alpha(HIF-2α) is an important regulator of chronic hypoxia, and thelate-stage renal tubular HIF-2α activation exerts protective effectsagainst renal fibrosis. However, its specific role in progressiverenal fibrosis remains unclear. Here, we investigated the effectsof the long-term tubular activation of HIF-2α on renal functionand fibrosis, using in vivo and in vitro models

고요산혈증의 치료는 전통적으로 요산 생성을 억제하는 전략에 초점을 맞추어 왔으나, 요산 배설 저하가 주된 병태로 작용하는 환자에서는 이러한 접근만으로 충분한 치료 효과를 얻기 어려운 경우가있다. 특히 만성신장질환(chronic kidney disease, CKD) 환자에서는 신장 기능 저하와 요산 배설 이상이동반되면서 고요산혈증의 임상적 중요성이 더욱 강조되고 있다. 요산의 체내 항상성은 생성과 배설의 균형에 의해 유지되며, 신장은 요산 배설을 조절하는 주요 기관으로 작용한다. 특히 근위 세뇨관에서의 재흡수와 분비 과정은 다양한 요산 수송체에 의해 정교하게 조절되며, 이 중 urate transporter 1 (URAT1)은 요산 재흡수를 매개하는 핵심 수송체로 알려져 있다. 최근 요산 배설 저하형 고요산혈증의 병태생리에 대한이해가 심화되면서, URAT1 억제제는 요산 재흡수를 선택적으로 차단하여 요산 배설을 증가시키는 새로운치료 전략으로 주목받고 있다. 본 종설에서는 URAT1 억