강미란 교수

Missense somatic mutations in IDH1 gene affecting codon 132 have recently been reported in glioblastoma multiforme (GBM) and other gliomas. The recurrent nature of the IDH1 mutations in the same amino acid strongly suggests that the mutations may play important roles in the pathogenesis of glial tumors. The aim of this study was to see whether the IDH1 codon 132 mutations occur in other human cancers besides glial tumors. We also attempted to confirm the occurrence of the IDH1 mutations in GBM o

Mounting evidence indicates that alterations of autophagy processes are directly involved in the development of many human diseases, including cancers. Autophagy-related gene (ATG) products are main players in the autophagy process. In humans there are 16 known ATG genes, of which four (ATG2B, ATG5, ATG9B and ATG12) have mononucleotide repeats with seven or more nucleotides. Frameshift mutations of genes with mononucleotide repeats are features of cancers with microsatellite instability (MSI). I

To study the genetic variation of the HIV-1 strains prevalent in South Korea, we analyzed the nef sequences derived from 46 HIV-1-positive individuals living in various geographic regions in Korea. Phylogenetic analysis revealed four subtypes of HIV-1: A (3 patients), B (41 patients), D (1 patient), and a type that could not be clearly classified to any known subtype (1 patient). Thirty-five of the 41 Korean subtype B isolates formed a distinct monophyletic clade that is not related to any of th

Topoisomerase I adjusts torsional stress in the genome by breaking and resealing one strand of the helix through a transient covalent coupling between enzyme and DNA. Camptothecin, a specific topoisomerase I poison, traps this covalent intermediate, thereby damaging the genome. Here we examined the activity of topoisomerase I at telomeric repeats to determine whether telomere structures are targets for DNA damage. We show that topoisomerase I is catalytically active in cleaving the G-rich telome

Abstract Clove has been shown to extend the shelf life of various foods. This study investigated whether it can prolong the shelf life of kimchi paste. Clove powder at concentrations of 0%, 0.5%, 1%, 1.5%, and 2% was added to kimchi paste, which was then sealed and stored at 10°C for 20 days. Changes in microbial counts, gas composition, sugar and organic acid contents, pH , titratable acidity, and reducing sugar content were evaluated. Adding clove powder inhibited the growth of total aerobic a

This study was aimed at evaluating the effect of red-yeast-rice supplementation on cholesterol-lowering and glucose control in subjects with impaired fasting glucose (IFT) or impaired glucose tolerance (IGT). We conducted a doubleblind, placebo-controlled study with 3 groups ; placebo, low dose group (red yeast rice 210.0 mg/capsule, 2.52 g/day) and high dose group (red yeast rice 420.0 mg/capsule, 5.04 g/day), which were randomly assigned to subjects with impaired fasting glucose or impaired gl

To consistently disinfect fresh vegetables efficiently, the decay of disinfectants such as chlorine, electrolyzed oxidizing water (EOW), ozonated water, and plasma-activated water during the disinfection maintenance stage needs to be understood. The aim of our study was to evaluate the changes in the inactivation kinetics of slightly acidic electrolyzed water (SAEW) against human norovirus (HuNoV), based on the cabbage-to-SAEW ratio. After disinfection of fresh cabbage with disinfected SAEW solu

Abstract Cross‐contamination of Pectobacterium carotovorum subsp. carotovorum (PCC) from a stainless‐steel surface to cabbage ( Brassica rapa L. subsp. pekinensis ) was evaluated. To investigate the PCC transfer pattern from mechanical knife surfaces to cabbage during 100 cuts, two mathematical models (power and logarithmic model) were fitted to the mean log 10 detection data from cabbage. Overall, regression analysis determined that the best‐fitting regression curves of planktonic cells and det

This study investigated changes in the quality and headspace O2/CO2 concentrations of young radish (Raphanus sativus L.) kimchi (YR-kimchi) packaged using a multilayer (ML) film with or without sachets containing a CO2 absorber and nano-foamed structure (NFS) film, respectively, during storage at −2.2 or 4.0°C. Compared to that in YR-kimchi samples stored at 4.0°C, the total lactic acid bacteria count and titratable acidity of YR-kimchi samples stored at −2.2°C increased rapidly until 21 days of

열무김치로부터 김치유산균을 분리하고 프로바이오틱스로 이용 가능한 균주를 선발하기 위하여 인체의 장관과 유사한 인체장모델시스템(SHIMS)을 이용한 in silico 실험을 수행하였다. 열무김치에서 분리한 5종과 표준균주 12종에 대해 내산성 및 내담즙산성을 시험하여 생존율이 높은 2종의 균주를 선발하였다. 선발한 균주를 SHIMS에서 시험하여 가장 높은 생존율을 나타낸 균주의 16S rRNA를 분석한 결과 Leu. mesenteroides로 동정되었으며 이 김치유산균을 Leu. mesenteroides K01으로 명명하였다. 이 균주는 SHIMS에서 생존율이 높았기 때문에 프로바이오틱스로 다양하게 이용할 가치가 있다고 판단된다.