윤승규 교수

Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes coughing in most patients, which possibly increases the risk of nosocomial spread of viruses. In this study, paired NPS and saliva specimens were collected from 236 adult male patient

Whole-cell-based therapy has been extensively used as an effective disease treatment approach, and it has rapidly changed the therapeutic paradigm. To fully accommodate this shift, advances in genome modification and cell reprogramming methodologies are critical. Traditionally, molecular tools such as viral and polymer nanocarriers and electroporation have been the norm for internalizing external biomolecules into cells for cellular engineering. However, these approaches are not fully satisfacto

Four different rapid diagnostic tests (RDTs) for malaria were evaluated by testing 82 healthy control patients, 89 Plasmodium vivax-infected patients, and 92 rheumatoid factor (RF)-positive nonmalaria patients. The false-positive rate ranged from 2.2% to 13% in RF-positive patients. High RF levels are associated with malaria RDT false positivity.

The cluster of differentiation 47 (CD47) protein is abundantly expressed on various malignant cells and suppresses the phagocytic function of macrophages and dendritic cells. High CD47 expression levels are correlated with poor cancer survival. Antagonizing CD47 antibodies with potent antitumor effects have been developed in clinical trials, but have critical side effects, inducing anemia and thrombocytopenia. To develop a safe and potent CD47 blockade, we designed extracellular vesicles (EVs) h

There have been few studies that comprehensively evaluated factors affecting immune responses and the correlation between humoral and cellular immune responses after vector, mix-and-match, and mRNA vaccines against SARS-CoV-2. Therefore, we analyzed the effects of age, sex, and the different vaccine regimens on the immune responses to vaccination against SARS-CoV-2. The correlation between humoral and cellular immune responses and the cutoffs were derived for RBD antibodies and neutralizing anti

BACKGROUND: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. METHODS: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three

eISSN 2093-6338 와 거핵구 생착을 지연시킬 수 있다[1]. 따라서 ABO 혈액형의 동종응집소 역가는 ABO 부적합 골수이 식 또는 장기 이식에서의 경과 관찰 및 예후 평가, ABO 혈액형의 아형에 대한 검사, ABO 혈액형 부적합 수혈 시 반응에 대한 관찰 등에 도움을 줄 수 있다[2-5]. 특히 이식 후 거부반응의 예측에 중 요한 정보를 제공하여 혈장 교환술, 비장절제, 항-CD20 단클론성 항체, 면역글로불린 정주요법 등과 같은 치료방법의 선택에 도움 을 줄 수 있는데[6], 인종이나 연령간 유의한 차이를 보일 수 있을 뿐 아니라, 검사법에 따라서 많은 영향을 받을 수 있다. ABO 동종응집소는 측정방법에 따라 시험관법, 미세원주응집 법, 효소면역법, 유세포분석법 등 다양하게 측정될 수 있으며 이

Practical applications of innovative host-guest systems are challenging because of unexpected guest competitors and/or subtle environmental differences. Herein, a supramolecular mass spectrometry (MS)-based method using a synthetic host, cucurbit[7]uril (CB[7]), was developed for identifying and quantifying N-glycolylneuraminic acid (Neu5Gc) in therapeutic glycoproteins, which critically reduces drug efficacy. The development of a reliable derivatization-free analytical method for Neu5Gc is high

BACKGROUND: Prognosis of patients with diverse chronic diseases is reportedly associated with 25-hydroxyvitamin D levels. In this study, we investigated the potential role of 25-hydroxyvitamin D3 (25[OH]D3) levels in improving the predictive power of conventional prognostic models for patients with liver cirrhosis. METHODS: We investigated clinical findings, including serum 25(OH)D3 levels at admission, of 155 patients with cirrhosis who were followed up for a median of 16.9 months. RESULTS: Med

BACKGROUND: The seroprevalence rate of human T-lymphotropic virus I and II (HTLV-I/II) in Korean blood donors has been known as 0.004%, and HTLV-I/II Ab screening test has been performed since 2008 in Korea. Korea Ministry of Food and Drug Safety (MFDS) approved two chemiluminescent microparticle immunoassays (CMIA) for testing HTLV-I/II antibody, ABBOTT PRISM HTLV-I/HTLV-II and ARCHITECT rHTLV-I/II. A multicenter performance evaluation study in Europe and Japan was carried out with the new elec

Background: In Korea, a screening panel of cells from abroad without positive cells has been commonly used when a patient has an unexpected antibody screening test. It has been reported that occurs with a frequency of 6.14 to 14.5% among Koreans. However, the current popular antibody screening panels contain no positive cells. In this study, we evaluate the clinical usefulness of the Cell Panel (Diagnostic Grifols, Barcelona, Spain) for Koreans. Methods: A total of 3,372 pretransfusion samples w

Seung Gyu Yun, M.D., Dae-Hyun Ko, M.D., Young-Eun Kim, M.D., Kyoung Ho Roh, M.D., Jaehyeon Lee, M.D., Jin-Hee Cho, M.D., Misuk Ji, M.D., Ki Ho Hong, M.D., Hyung-Doo Park, M.D., Gye Cheol Kwon, M.D.. Lab Med Online 2021;11:217-22. https://doi.org/10.47429/lmo.2021.11.4.217

BACKGROUND: Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance. In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV). METHODS: The Hi3-1 assay was tested using four panels (Panel 1, n = 4,581 patient samples; Panel 2, n = 15