윤선옥 교수

The tumor-associated glycoprotein (TAG)-72 is expressed in the majority of human adenocarcinomas but is rarely expressed in most normal tissues, which makes it a potential target for the diagnosis and therapy of a variety of human cancers. Here we describe the construction, affinity maturation, and biological characterization of an anti-TAG-72 humanized antibody with minimum potential immunogenicity. The humanized antibody was constructed by grafting only the specificity-determining residues (SD

In a previous study we generated an anti-Hepatitis B Virus (HBV) preS1 humanized antibody (HzKR127) that showed in vivo HBV-neutralizing activity in chimpanzees. However, the antigen-binding affinity of the humanized antibody may not be sufficient for clinical use and thus affinity maturation is required for better therapeutic efficacy. In this study, phage display technique was employed to increase the affinity of HzKR127. All six amino acid residues (Glu95-Tyr96-Asp97-Glu98-Ala99-Tyr100) in th

Selection and maturation of B cells into plasma cells producing high-affinity antibodies occur in germinal centers (GC). GCs form transiently in secondary lymphoid organs upon antigen challenge, and the GC reaction is a highly regulated process. TGF-β is a potent negative regulator, but the influence of other family members including bone morphogenetic proteins (BMPs) is less known. Studies of human peripheral blood B lymphocytes showed that BMP-6 suppressed plasmablast differentiation, whereas

Background and Objectives:Conventional turbinoplasty and laser-assisted partial turbinoplasty (LAPT are commonly performed to control nasal obstruction secondary to inferior turbinate hypertrophy. Coblation, one of radiofrequency electro- surgeries, has recently been introduced as a new treatment modality for turbinate surgery. The aim of the current study is to compare the postoperative outcome of Coblation-assisted partial turbinoplasty (CAPT with that of LAPT. Materials and Method:Forty-one p

Purpose The aims of this study are to find out whether the sequence of chemotherapeutic regimens including second- and third-line taxane and irinotecan influences the survival of patients with unresectable gastric carcinoma and to identify clinical characteristics of patients with improved response. Materials and Methods Fifty gastric carcinoma patients who were treated by third-line sequential chemotherapy between November 2004 and July 2010 were enrolled in this study. Their overall survival (

Background: The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material is necessary. Methods: Pairs of bilateral bone marrow biopsies sampled from 53 patients were decalcified according to protocols of two comparison groups: EDTA versus HCl and RDO GOLD (RDO) versus HC

We report a case of an Epstein-Barr virus (EBV)-associated inflammatory pseudotumor-like follicular dendritic cell tumor (IPT-like FDC tumor). The tumor occurred in the spleen of a 64- year-old woman with a history of a diffuse large B-cell lymphoma (DLBCL) of neck nodes that presented four years ago. The splenectomy specimen revealed a 5 cm-sized, tan-colored and well-circumscribed mass. Histologically, spindle or ovoid cells with large vesicular nuclei were admixed with abundant inflammatory c

Purpose: To assess the diagnostic potential of whole-exome sequencing (WES) and elucidate the clinical and genetic characteris tics of primary ciliary dyskinesia (PCD) in the Korean population. Materials and Methods: Forty-seven patients clinically suspected of having PCD were enrolled at a tertiary medical center. WES was performed in all patients, and seven patients received biopsy of cilia and transmission electron microscopy (TEM). Results: Overall, PCD was diagnosed in 10 (21.3%) patients:

목적: 호산구 식도염(eosinophilic esophagitis, EoE)은 식도의 과도한 호산구 침윤으로 인하여 연하 곤란 등의 증상을 일으키는 드문 질환이나, 최근 유병률이 증가하고 있다. 그러나 아직도 이에 대한 임상적, 병리적 인지 부족으로 인하여 진단이 적절히 이루어지지 않고 있다. 저자들은 비폐쇄성 연하곤란(nonobstructive dysphagia, NOD) 환자의 임상 증상, 내시경, 식도 조직의 병리 소견과의 비교를 통하여 EoE의 내시경 소견 및 임상병리적 특징을 고찰하였다. 대상 및 방법: 2006년 6월부터 2010년 10월까지 강남세브란스 병원에서 내시경을 받은 환자들 중, EoE로 진단된 12명과 연하곤란으로 식도 조직검사를 받은 NOD 13명을 대상으로 하였다. EoE의 진단은 식도 조직검사 상 고배율 시야 당 호산구가 20개 이상 관찰되는 것으로 하였으며, 양 군의 내시경 소견과 병리 슬라이드를 후향적으로 재검토하여 병리 소견을 비교하였다. 결과: E

Struma ovarii is defined as a rare monodermal form of ovarian teratoma that occupies more than 50% of thyroid tissue. Only 2% of struma ovarii display malignant histology, most of which is revealed as papillary carcinoma type. Follicular carcinoma originating from malignant struma ovarii is rarely reported. We report a case of a pregnant woman diagnosed with peritoneal dissemination of follicular carcinoma arising from struma ovarii.

Background: The Bethesda System for Reporting Thyroid Cytopathology (BSRTC) uses six diagnostic categories to standardize communication of thyroid fine-needle aspiration (FNA) interpretations between clinicians and cytopathologists. Since several studies have questioned the diagnostic accuracy of this system, we examined its accuracy in our hospital. Methods: We calculated the incidences and malignancy rates of each diagnostic category in the BSRTC for 1,730 FNAs that were interpreted by four cy