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A mathematical model based on known molecular interactions has been formulated to describe quantitatively regulation of expression of the lactose (lac) operon in the Escherichia coli chromosome and in multicopy plasmids. This model is genetically structured such that a nucleotide sequence change affecting transcription initiation at the lac promoter-operator influences one or very few directly corresponding model parameters. The model simulates chromosomal lac operon function in good agreement w

The influence of growth rate on Escherichia coli plasmid content and expression of a cloned-gene product has been described by a mathematical model based upon the molecular mechanism of lambdadv plasmid replication and known relationships between growth rate and transcription and translation activities of the host cell. The model simulates correctly decreases in plasmid content with increasing growth rate as observed experimentally for pBR322, NR1, R1, and Col E1 plasmids. A maximum with respect

A mathematical model has been formulated for product formation by unstable recombinant organisms in batch and in continuous flow reactors. The cell population is characterized by three different genotypes according to the absence or presence of plasmids (segregational instability) and of active cloned gene (structural instability). Empirical growth inhibition factors due to plasmids and to product protein are assigned to the corresponding strains. Product formation kinetics are based upon a quas

A mathematical model based on molecular mechanisms for regulation of the lactose (lac) operon in Escherichia coli has been extended and applied to investigate the lac promoter function in the chromosome and in multicopy plasmids. The model simulates the influence of certain host cell mutations and also mutations in the lac promoter sequence in the chromosome in reasonable agreement with previous experimental measurements. The effect of the plasmid copy number and the cloning vector size on the p

Aptamers are functional nucleic acids that can specially bind to proteins, peptides, amino acids, nucleotides, drugs, vitamins and other organic and inorganic compounds. The aptamers are identified from random DNA or RNA libraries by a SELEX (systematic evolution of ligands by exponential amplification) process. As aptamers have the advantage, and potential ability to be released from the limitations of antibodies, they are attractive to a wide range of therapeutic and diagnostic applications. A