최성일 교수

While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated by interaction with RNA. When an RNA-binding module is placed at the N-terminus of aggregation-prone target proteins, this module, upon binding with RNA, further promotes the solubility of passenger proteins, potentially leading to enhancement o

Traditionally the principles of protein folding in vivo have been obtained largely from molecular chaperone studies. Through extensive studies on molecular chaperones, it becomes clear that most proteins can fold without their assistance in vivo, suggesting the existence of other chaperone types and mechanisms. Since all nascent polypeptides are linked to the ribosomes, protein folding in vivo should be understood in the context of vectorial protein synthesis and linkage of nascent chains to rib

국내 커피 시장은 경제 불황에도 불구하고 가파른 성장세를 보이고 있으며, 불황의 영향을 크게 받지 않는 산업으로 급부상하고 있다. 최근 2~3년 사이에 커피전문점의 점포수가 기하급수적으로 늘어나며 포화상태에 이르렀다는 지적도 나오고 있지만 커피전문점 시장의 성장세는 당분간 지속될 것으로 전문가들은 판단하고 있다. 본 연구는 커피전문점 선택속성에 대한 분석을 통해 현재 커피 시장의 시장점유율을 측면에서 경쟁우위에 있는 커피전문점의 차별화된 선택속성을 확인하여 소비자태도를 분석하고 소비자의 커피전문점 구매행동의도를 연구한 결과, 커피전문점 선택속성에 대한 IPA결과 CS포트폴리오 상에 1사분면에 위치하여 좋은 성과를 지속적으로 유지해야 하는 항목은 서비스 품질, 커피품질, 물리적 시설이다. 2사분면에 위치하여 적극적으로 개선해야 할 항목은 접근성이고, 3사분면에 위치하여 점진적으로 개선해야 할 항목은 판촉 이벤트, 메뉴 다양성이며, 4사분면에 위치하여 지속적으로 유지해야 할 항목은 브랜

The purpose of study is to identify the selective attributes of coffee shop and to verify the effects of those attributes on perceived value and customer loyalty. We categorized the selective attributes of coffee shop as seven dimensions (i.e., coffee quality, menu variety, physical facilities, brand, service quality, sales promotion). We considered perceived value as two parts such as functional and emotional. We also specified two segments of customer loyalty as intention of re-visit and recom

The fusion of soluble partner to the N terminus of aggregation-prone polypeptide has been popularly used to overcome the formation of inclusion bodies in the E. coli cytosol. The chaperone-like functions of the upstream fusion partner in the artificial multidomain proteins could occur in de novo folding of native multidomain proteins. Here, we show that the N-terminal domains of three E. coli multidomain proteins such as lysyl-tRNA synthetase, threonyl-tRNA synthetase, and aconitase are potent s

This study developed and tested a model of the effects of family-friendly corporate policies on employees' outcomes. To test the conceptual model and hypotheses, data were collected from 363 workers employed in a variety of organizations spanning many industries in Korea. Amos was used to test a model of path effects of family-friendly policies on employees' work-family balance, human resource outcomes(job satisfaction, turnover intentions, and work performance), and quality of life. The results

Protein folding in vivo is extremely intricate and challenging to examine or predict because the conformational changes, including folding, misfolding, and aggregation, are largely influenced by the cellular environment. Traditionally, cellular protein folding has been considered predominantly in the context of the Anfinsen postulate and molecular chaperones. However, accumulating evidence reveals that these models have limitations. In this review we revisit these models, and discuss co-translat

BACKGROUND: Despite an association between visit-to-visit blood pressure (BP) variability (VV-BPV) and cardiovascular (CV) outcomes, many studies performed during the past 4 years have shown conflicting results. This study investigated the impact of VV-BPV on CV outcomes in the Korean National Health Insurance Service (NHIS) database-National Sample Cohort. METHODS: From the 2002 Korean NHIS database (n = 47,851,928), sample subjects with 3 or more BP measurements (n = 51,811) were divided into

The purpose of this study is to introduce the evolution of corporate family-friendly policies and programs, a recent business issue throughout the world. In addition, this study suggests implications for a corporate reference guide to work-family policies and programs by providing a comparative analysis of the typical stages of development of U.S. corporate family-friendly policies and programs, and by presenting model initiatives of Korean and American family-friendly companies. Just as technol

A few studies have compared auscultation and oscillometric devices with the mercury sphygmomanometer (MS) reference values for blood pressure (BP) measurement in an epidemiologic survey.Four trained observers recorded BP measurements from 766 subjects from general Korean population in 2014 and 2015. Measurements were repeated 3 times for each device, alternately using an MS and 2 electronic devices (EDs; Greenlight 300 vs Omron HEM-907), together with a randomized device sequence. The BP measure

BACKGROUND: Carotid intima-media thickness (CIMT) and carotid atherosclerotic plaque (CAP) are well-known indicators of atherosclerosis. However, few studies have reported the value of CIMT and CAP for predicting renal artery stenosis (RAS). We investigated the predictive value of CIMT and CAP for RAS and propose a model for predicting significant RAS in patients undergoing coronary angiography (CAG). METHODS: Consecutive patients who underwent renal angiography at the time of CAG in a single ce

The principles obtained from studies on molecular chaperones have provided explanations for the assisted protein folding in vivo. However, the majority of proteins can fold without the assistance of the known molecular chaperones, and little attention has been paid to the potential chaperoning roles of other macromolecules. During protein biogenesis and folding, newly synthesized polypeptide chains interact with a variety of macromolecules, including ribosomes, RNAs, cytoskeleton, lipid bilayer,