조율희 교수

To generate new scaffold candidates as highlyselective and potent cyclin-dependent kinase (CDK) inhibitors,structure-based drug screening was performed utilizing 3Dpharmacophore conformations of known potent inhibitors. Asa result, CR229 (6-bromo-2,3,4,9-tetrahydro-carbolin-1-one)was generated as the hit-compound. A computational dockingstudy using the X-ray crystalographicstructure of CDK2 incomplex with CR229 was evaluated. This predicted bindingmode study of CR229 with CDK2 demonstrated that

In the course of screening for a novel inhibitorof CDC2, HY558-1 was isolated from a culture broth ofPenicillium minioluteum F558. Moreover, it was found thatHY558-1 had an effect on both the cell cycle regulation andapoptosis of human cervical adenocarcinoma HeLa cells. Aflow cytometric analysis of HeLa cells revealed appreciablecell cycle arrest at the G1 and G2/M phases following treatmentwith HY558-1. Furthermore, DNA fragmentation due toapoptosis was observed in HeLa cells treated with HY55

In the search for a novel cytotoxic substance frommedicinal plants, HY53 (C17H32O2N2; molecular weight 296)was isolated from the leaves of Pata de Vaca (Bauhiniaforficatadose-dependent maner when treated with 0.07 to 0.40 mMHY53 for 24 h (IC50: 0.13 mM). Furthermore, nuclear DAPIstaining revealed the typical nuclear features of apoptosis inthe HepG2 cells exposed to 0.27 mM HY53, whereas a flowcytometric analysis of the HepG2 cells using propidium iodideshowed that the apoptotic cel population i

Chromosome microdissection and the reverse FISHtechnique is one of the most useful methods for the identificationof structurally abnormal chromosomes. In particular, the lasermicrobeam microdissection (LM) method alows rapidisolation of a target chromosome or a specific region ofcontamination. Isolated chromosomes were directly amplifiedby the degenerate oligonucleotide-primed polymerase chainreaction (DOP-PCR), and then the FISH probes labeled withspectrum green- or spectrum red-dUTP were gener

We report on a de novo centric fission of chromosome 11 in a healthy female referred for chromosome analysis due to recurrent miscarriages. Both fission products were mitotically stable. This centric fission of chromosome 11 appears to have no clinical significance for this patient other than recurrent miscarriages.

Purpose: We evaluated the effect of human parathyroid hormone (hPTH) on the engraftment and/or in vivo expansion of hematopoietic stem cells in an umbilical cord blood (UCB)-xenotransplantation model. In addition, we assessed its effect on the expression of cell adhesion molecules. Materials and Methods: Female NOD/SCID mice received sublethal total body irradiation with a single dose of 250 cGy. Eighteen to 24 hours after irradiation, 1×107 human UCB-derived mononuclear cells (MNCs) and 5×106 h

We have analyzed 15 short tandem repeat (STR) markers included the Power Plex-16 (Promega) kit in a sample of 165 unrelated individuals from Korforensic parameters were calculated to provide an expanded and reliable forensic database. The Exact Test demonstrated that al loci surveyed here were found to be no deviation from Hardy-Weinberg equilibrium. Statistical analyses based on allele frequencies of the 15 STR loci show that twelve Eurasian populations tested can be clasified into two distinct

The quantitative fluorescent PCR (QF-PCR) assay for prenatal diagnosis of common chromosome aneuploidies introduced during the last few years. We report the first assessment of QF-PCR aneuploidy testing performed on a large Korean population. Blind prospective study was performed in 3700 amniotic fluid samples. All samples were analyzed by QF-PCR using with four STR markers located on chromosome 21 (D21S1435/D21S11/D21S1411/ D21S1412) and subsequently performed by conventional cytogenetic analys

Objectives: Data comparing results of off-pump and conventional surgery in octogenarians is very limited. Thus we chose to compare early adverse events between off-pump (OPCABG) and on-pump coronary artery bypass grafting (ONCABG) in patients above 80 years. Methods: A systematic review of multiple databases was performed to obtain original studies fulfilling search criteria. End-points (early mortality, stroke, respiratory failure, atrial fibrillation and myocardial infarction) were compared be

Purpose: CYP2D6 and N-acetyltransferase (NAT2) are polymorphic enzymes which are expressed in the hepatocyte in a genotype-determined manner. They are known to be involved in the inactivation and activation of various mutagens and carcinogens, respectively. The activities of the two enzyme systems are associated with the genetic susceptibility of many human cancers. Methods: This study was performed to determine the genotype frequencies of the two enzyme systems in primary hepatocellular carcino

DNA methylation is one of the major epigenetic regulations of gene expression. The DNA methylation patterns are dramatically changed during gametogenesis and embryogenesis, and especially, it has been known that embryonic stem cells show a distinct methylation pattern. In this study, we examined the methylation patterns of imprinting genes, H19, Igf2r, and Snrpn, in stem cells induced from fertilized embryo (fES) and somatic cell nuclear transferred embryo (ntES). The methylation pattern of H19

Aims and Method : Comparative genomic hybridization serves as a screening test for regions of copy number changes in tumor genomes. I have applied the technique to map DNA losses and gains in 13 cases of frozen cholangiocarcinomas. Results : All of the 13 cases showed genetic alterations. Loss of short arm of chromosome 19 (92%) was the most common changes observed. 22q(62%), 1p(54%), 17p(54%) and 19q(54%) also showed nonrandom patterns of genomic losses with high frequencies. Among the genomic

Objective: The history of gestational diabetes (GDM) is a high risk for the development of type 2 diabetes mellitus (T2DM). The purpose of this study is to investigate the genetic association of LEP and LEPR gene polymorphisms and the development of T2DM in Korean women of history of GDM. Methods: Women diagnosed as GDM during pregnancy from January 1992 to December 2002 were recruited. Those women with a T2DM at the time of study were classified as T2DM positive group, and without T2DM, as T2DM